The function of Fmp12, which has a similarity with α-ketoglutarate-dependent dioxygenases of the yeast Candida species and human, might inhibit cell growth by skipping the ATP production step of the TCA cycle. These results provided the first evidence that proline can be utilized as a carbon source via the mitochondrial proline metabolic pathway and the subsequent tricarboxylic acid (TCA) cycle in S. Deletion of the genes that encode mitochondrial enzymes, such as proline dehydrogenase ( PUT1), Δ 1-pyrroline-5-carboxylate dehydrogenase ( PUT2), alanine transaminase ( ALT1), and α-ketoglutarate dehydrogenase subunit ( KGD1), abolished the enhanced cell growth in Δ fmp12.

The Fmp12 protein was localized in the mitochondria and was constitutively expressed. In contrast, overexpression of FMP12 negatively affected cell growth under the same condition.

In the process of study on the physiological roles of the found-in-mitochondrial-proteome ( FMP) genes in proline metabolism, we found that Δ fmp12 cells could grow better than wild-type cells on agar plate medium containing proline as the sole nitrogen and carbon sources. cerevisiae cells has not been studied yet. However, utilization of proline as a carbon source in S.

The amino acid proline functions as a nitrogen source and as a stress protectant in the yeast Saccharomyces cerevisiae.

Fmp 12